Normal Human Kidney Mesangial Cells
人肾小球系膜细胞

Normal Human Kidney Mesangial cells (NhKM) are found within the glomerulus and are derived from the cortex of the kidney during the isolation process. These cells “fill the gap” between adjacent capillary loops surrounded by the glomerular basement membrane (GBM) and the podocytes. The primary function of NhKM is to remove trapped residues and aggregated protein from the basement membrane.  In addition, they aid filtration by constituting part of the glomerular capillary tuft structure that filters fluids to produce urine.

The NhKM cells are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhKM cells are isolated by PDGF-R beta positive selection as a pure population, then cryopreserved.

Purity is analyzed using immunofluorescence microscopy for PDGFRβ, vimentin, and α smooth muscle actin. Cells are terminally differentiated and can be expanded for at least 15 population doublings. Mesangial cells have an intermediate phenotype, sharing features of fibroblasts and smooth muscle cells.

When grown in one of the common media formulations for eukaryotic cells (e.g. MEM, DMEM, Waymouth, RPMI 1640), these cells retain their in vivo phenotype as stellate, arborized elements in monolayer culture. Homogeneous cultures of Mesangial cells resemble smooth muscle cells or fibroblasts. On average, 15–20 passages in culture can been obtained from NhKM cells.

The NhKM cells are terminally differentiated.

THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.

PBMC分离试剂盒
人外周血单个核细胞分离试剂盒PBMC分离液
人PBMC分离试剂盒 人PBMC分离液
猴外周血单个核细胞分离试剂盒PBMC分离液
猴PBMC分离试剂盒 猴PBMC分离液
比格犬外周血单个核细胞分离试剂盒PBMC分离液
比格犬PBMC分离试剂盒 比格犬PBMC分离液
狗外周血单个核细胞分离试剂盒PBMC分离液
狗PBMC分离试剂盒 狗PBMC分离液
大鼠外周血单个核细胞分离试剂盒PBMC分离液
大鼠PBMC分离试剂盒 大鼠PBMC分离液
小鼠外周血单个核细胞分离试剂盒PBMC分离液
小鼠PBMC分离试剂盒 小鼠PBMC分离液
兔外周血单个核细胞分离试剂盒PBMC分离液
兔PBMC分离试剂盒 兔PBMC分离液
猪外周血单个核细胞分离试剂盒PBMC分离液
猪PBMC分离试剂盒 猪PBMC分离液
豚鼠外周血单个核细胞分离试剂盒PBMC分离液
豚鼠PBMC分离试剂盒 豚鼠PBMC分离液
骨髓单个核细胞分离试剂盒Bone Marrow Mononuclear Cells Isolation Kit
脾单个核细胞分离试剂盒 Spleen Mononuclear Cells Isolation Kit

红细胞分离试剂盒
人红细胞分离试剂盒 红细胞分离液
猴红细胞分离试剂盒 红细胞分离液
比格犬红细胞分离试剂盒 红细胞分离液
大鼠红细胞分离试剂盒 红细胞分离液
小鼠红细胞分离试剂盒 红细胞分离液

ADME相关产品
Ⅰ相代谢稳定性试剂盒
Ⅱ相代谢稳定性试剂盒
CYP450 酶代谢表型研究试剂盒（化学抑制法/7种抑制剂）
CYP450 酶代谢表型研究试剂盒（重组酶法/7种酶）
CYP450 酶代谢表型研究试剂盒（重组酶法/单酶）
酶抑制（IC50）研究试剂盒（7种特异性底物）
酶抑制（IC50）研究试剂盒（单个酶）
NADPH再生系统
UGT孵育系统
0.1M PBS
肝微粒体（人，猴，犬，大鼠，小鼠）
肝S9（人，猴，犬，大鼠，小鼠）
肝原代细胞（人，猴，犬，大鼠，小鼠）
CYP450（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）
UGT酶
探针底物，代谢产物，抑制剂（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

ADME服务项目
肝微粒体代谢稳定性（人，猴，犬，大鼠，小鼠）
肝细胞代谢稳定性试验（人，猴，犬，大鼠，小鼠）
代谢表型研究
代谢产物鉴定
代谢途径鉴定
种属比较研究
CYPP450抑制实验（CYP1A2，CYP2A6，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）
CYPP450诱导实验
血浆蛋白结合率测定
血浆稳定性试验
跨膜转运试验
药物-药物相互作用
毒理学研究

Innovative Reagents for Innovative Research
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