Normal Human Kidney Podocytes
人肾足细胞
Normal Human Kidney Podocytes (NhKP) are terminally differentiated cells found lining the outer surface of the glomerular capillaries.
The NhKP are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhKP are isolated by nephrin and podocin positive selection as a pure population, then cryopreserved.
The podocytes have foot processes, that form the slit diaphragm to allow ultrafiltration of the blood based on size and charge and allows the filtration of…, electrolytes, and small and midsized solutes while restricting the passage of anionic molecules and macromolecules.
Expression of slit diaphragm molecules nephrin and podocin will be tested by immunostaining and qPCR. Absence of CD-31 and PDGFβ-R (endothelia and mesangial markers, respectively) will be assessed by immunofluorescence and qPCR.
Podocytes cannot be cultured for more than 1 or 2 passage without loss of their particular traits (like foot processes and expression of slit diaphragm proteins).
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.
PBMC分离试剂盒
人外周血单个核细胞分离试剂盒PBMC分离液
人PBMC分离试剂盒 人PBMC分离液
猴外周血单个核细胞分离试剂盒PBMC分离液
猴PBMC分离试剂盒 猴PBMC分离液
比格犬外周血单个核细胞分离试剂盒PBMC分离液
比格犬PBMC分离试剂盒 比格犬PBMC分离液
狗外周血单个核细胞分离试剂盒PBMC分离液
狗PBMC分离试剂盒 狗PBMC分离液
大鼠外周血单个核细胞分离试剂盒PBMC分离液
大鼠PBMC分离试剂盒 大鼠PBMC分离液
小鼠外周血单个核细胞分离试剂盒PBMC分离液
小鼠PBMC分离试剂盒 小鼠PBMC分离液
兔外周血单个核细胞分离试剂盒PBMC分离液
兔PBMC分离试剂盒 兔PBMC分离液
猪外周血单个核细胞分离试剂盒PBMC分离液
猪PBMC分离试剂盒 猪PBMC分离液
豚鼠外周血单个核细胞分离试剂盒PBMC分离液
豚鼠PBMC分离试剂盒 豚鼠PBMC分离液
骨髓单个核细胞分离试剂盒Bone Marrow Mononuclear Cells Isolation Kit
脾单个核细胞分离试剂盒 Spleen Mononuclear Cells Isolation Kit
红细胞分离试剂盒
人红细胞分离试剂盒 红细胞分离液
猴红细胞分离试剂盒 红细胞分离液
比格犬红细胞分离试剂盒 红细胞分离液
大鼠红细胞分离试剂盒 红细胞分离液
小鼠红细胞分离试剂盒 红细胞分离液
ADME相关产品
Ⅰ相代谢稳定性试剂盒
Ⅱ相代谢稳定性试剂盒
CYP450 酶代谢表型研究试剂盒(化学抑制法/7种抑制剂)
CYP450 酶代谢表型研究试剂盒(重组酶法/7种酶)
CYP450 酶代谢表型研究试剂盒(重组酶法/单酶)
酶抑制(IC50)研究试剂盒(7种特异性底物)
酶抑制(IC50)研究试剂盒(单个酶)
NADPH再生系统
UGT孵育系统
0.1M PBS
肝微粒体(人,猴,犬,大鼠,小鼠)
肝S9(人,猴,犬,大鼠,小鼠)
肝原代细胞(人,猴,犬,大鼠,小鼠)
CYP450(CYP1A2,CYP2A6,CYP2B6,CYP2C8,CYP2C9,CYP2C19,CYP2D6,CYP2E1,CYP3A4)
UGT酶
探针底物,代谢产物,抑制剂(CYP1A2,CYP2A6,CYP2B6,CYP2C8,CYP2C9,CYP2C19,CYP2D6,CYP2E1,CYP3A4)
ADME服务项目
肝微粒体代谢稳定性(人,猴,犬,大鼠,小鼠)
肝细胞代谢稳定性试验(人,猴,犬,大鼠,小鼠)
代谢表型研究
代谢产物鉴定
代谢途径鉴定
种属比较研究
CYPP450抑制实验(CYP1A2,CYP2A6,CYP2C9,CYP2C19,CYP2D6,CYP2E1,CYP3A4)
CYPP450诱导实验
血浆蛋白结合率测定
血浆稳定性试验
跨膜转运试验
药物-药物相互作用
毒理学研究
Innovative Reagents for Innovative Research
汇智和源致力于为创新药企业及生命科学研究机构提供高品质的生物试剂。