Catalogue Number:                     CYP/EZ009
Product Description:                     Human CYP2E1 and human CYP-reductase coexpressed in Escherichia coli
Amount:                                    0.5 nmol cytochrome P450
Storage Buffer:                           50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage:                                    Store at -80oC. Avoid frequent temperature changes. Thaw on ice.
Intended Use:                             For laboratory (research) purposes only.
Batch Number:                            C2E1R020
P450 Concentration:                      1.0 nmol/ml
Protein Concentration:                    10.0 mg/ml
Specific P450 Content:                    100 pmol/mg protein
Cytochrome c Reductase Activity:     300 nmol/min/mg protein

P450 Activity Data

Activity:                              Chlorzoxazone 6-hydroxylase
Linear with time up to:           12 min
Linear with P450 up to:          75 pmol/ml
Vmax:                                15 pmol/min/pmol CYP
Km:                                    407 μM

CYP450酶，CYP450酶代谢底物，代谢产物及相关抑制剂，可用于体外药物代谢稳定性、代谢表型等研究。体外代谢研究可直接观察候选化合物与受试靶点的选择性作用，不需要消耗大量的样品和实验动物，操作快速简便，适用于代谢信息不明确的候选化合物进行高通量筛选。