PRODUCT DETAILS |
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Product Name | Anti-TSLP antibody | |
Species Reactivity | Human, Primate | |
Source | Recombinant chimeric antibody (mouse) | |
Isotype | IgG1-kappa (human) | |
Expression System | HEK 293 cell | |
Purification | Protein A purified | |
Label | Unconjugated | |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose | |
Reconstitution |
Centrifuge vial prior to opening vial. Add 0.5 mL sterile water or 0.01M PBS (pH7.2) at 0.4 mg/mL. Gently pipet solution down the sides of the vial. DO NOT VORTEX. |
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Please note: For research use only. Not for diagnostic or therapeutic use. | ||
SHIPPING & STROAGE |
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Shipping | Lyophilized product is shipped at ambient temperature. | |
Storage |
Upon receipt, store immediately at -20 to -70°C:12 months from date of receipt. After reconstitution under sterile conditions: 2 to 8 °C or 1 month or -20 to -70°C for 6 months. Avoid freeze-thaw cycles. |
Item | Description | Data Examples |
Immunoassay |
Ligand Binding-Sandwich ELISA In a sandwich ELISA, a concentration of 1 μg/mL (100 μL/well) of recombinant human TSLP (Cat.#: 0721061) will be detected by the Anti-TSLP Ab (Cat.#: 0731062) when used with goat anti-human IgG(H+L) as the secondary antibody. The EC50 is 92 ng/mL. |
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Neutralization |
Inhibiting STAT-5 Signaling The stimulation of STAT5-Luciferase expression by the recombinant human TSLP (Cat.#: 0721061) in the HEK293 cells (Cat.#: 0611142) is neutralized by the Anti-TSLP antibody (Cat.#: 0731062) in a dose-dependent manner. The ND50 is typically 6.21 μg/mL. |
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Blocking TSLPR binding In a sandwich ELISA, a concentration of 1μg/mL (100 μL/well) of anti-TSLP Ab (Cat.#: 0731062) will block binding of recombinant human TSLPR (1 μg/mL) (Cat. #: 0721112) to the recombinant human TSLP (Cat.#: 0721061) in a dose-dependent manner. The ND50 is 1.49 μg/mL. |